Mot1 subfamily
The Mot1 subfamily derives its name from the S
cerevisiae Mot1 protein (Modifier of Transcription)
1. It encompasses
homologues with highly conserved helicase-like
regions present across fungi and all higher
eukaryotes, where it is known as BTAF1 or TAF172
2.
In vitro and in vivo studies of the yeast enzyme
suggest that Mot1p interacts intimately with TBP
3, probably acting
to recycle it from certain DNA-bound states 4. As such, Mot1p is
an example of a Snf2 family enzyme whose role is not
to manipulate nucleosome structure, although a
possible direct involvement with chromatin has also
been proposed 5.
names associated with subfamily members
TAFII170, TAF172, BTAF1, Hel89B
references
1: Davis, J. L., R.
Kunisawa, et al. (1992). A presumptive helicase
(MOT1 gene product) affects gene expression and
is required for viability in the yeast
Saccharomyces cerevisiae. Mol Cell Biol 12(4):
1879-92.
PubMed
2: Chicca, J. J., 2nd, D.
T. Auble, et al. (1998). Cloning and biochemical
characterization of TAF-172, a human homolog of
yeast Mot1. Mol Cell Biol 18(3): 1701-10.
PubMed
3: Auble, D. T., D. Wang,
et al. (1997). Molecular analysis of the
SNF2/SWI2 protein family member MOT1, an
ATP-driven enzyme that dissociates TATA-binding
protein from DNA. Mol Cell Biol 17(8): 4842-51.
PubMed
4: Dasgupta, A., S. A.
Juedes, et al. (2005). Mot1-mediated control of
transcription complex assembly and activity.
Embo J 24(9): 1717-29.
PubMed
5: Topalidou, I., M.
Papamichos-Chronakis, et al. (2004). Spt3 and
Mot1 cooperate in nucleosome remodeling
independently of TBP recruitment. Embo J 23(9):
1943-8.
PubMed
